Contact

Prof. Dr. Dr. Maximilian Emmert

Maximilian Y. Emmert, MD, PhD
Deutsches Herzzentrum der Charité (DHZC)
Department of Cardiothoracic and Vascular Surgery
Augustenburger Platz 1, 13353 Berlin, Germany
E-Mail: maximilian.emmert@dhzc-charite.de

In vitro methods

  • Isolation of cells from heart tissue, whole blood, and umbilical cords (e.g., mesenchymal stromal cells, fibroblasts, endothelial cells)
  • Cell culture: primary cells, cell lines, organoids, 3D microtissues
  • Bacterial culture
  • Gene transfer and genetic engineering: transfection, transduction
  • Isolation of DNA, RNA and proteins from cells, tissues and bacterial samples
  • Gene and protein expression analysis: PCR, qPCR, flow cytometry, Northern blot, Southern blot, Western blot
  • Microscopy: bright field, fluorescence, confocal, live cell imaging, high content imaging
  • Enzyme-linked immunosorbent assay, fluorescence in situ hybridization
  • Functional assays: cell toxicity, viability, autophagy, angiogenesis, senescence, proliferation
  • MicroRNA sequencing, 16S ribosomal DNA sequencing
  • Histology: Harvesting, processing, staining and conservation of heart tissue and saphenous vein grafts
  • Isolation and characterization of extracellular vesicles from various cell lines (e.g., nanoparticle tracking analysis)
  • Isolation of peripheral blood mononuclear cells from whole blood
  • Plasma surface technology for surface modification
  • In vitro simulation of ischemia/reperfusion

In vivo methods

  • Murine models of ischemia reperfusion injury (e.g., myocardial infarction, hind/limb ischemia)
  • Large animal models for regenerative medicine (e.g., intracoronary and intramyocardial delivery)
  • Large animal models of cardiovascular disease  (e.g., ischemic and structural heart disease and peripheral vascular disease)
  • Development of new models for cardiovascular disease and therapeutics (e.g., pigs and sheeps)
  • Intestinal bacterial colonization

Equipment

  • Hypoxia incubator, Binder
  • LightCycler 480 II 384 well, Roche
  • MACSQuant VYB, Miltenyi Biotec
  • Microscope BZ-X700, Keyence
  • Microplate reader Epoch 2, BioTek
  • Microplate washer 405 TS, BioTek
  • Parallel-plate flow chamber in an IF750 incubator, Memmert
  • Plasma surface technology, ZEPTO, Diener             
  • ZetaView, Particle Metrix
  • … and many more …

If you are interested in using equipment from our group, please do not hesitate to get in touch with Prof. Emmert. We welcome collaboration and look forward to a mutually beneficial exchange. Feel free to contact us to explore this opportunity.